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  High Resolution Fiber FISH:
  • Construct a high-resolution physical map for specific regions
  • Measure the physical distance between two markers
  • Determine the order of markers along chromosomes with centromere-telomere orientation
  • Determine the number of gene duplications/deletions in disease conditions
  • Analyze the gene amplification array
  • Map the translocation point
  • Confirm mapping data generated from a radiation hybrids panel
  • Determine the physical relationship between an identified novel gene and other genetic markers

    Fiber FISH
    Figure 4.

    A. High resolution FISH mapping on released free chromatin. The resolution of chromatin fiber mapping is approximately 20 Kb, while the coverage is 20Kb -- 5,000Kb.
    B. High resolution FISH mapping on released DNA fiber. The resolution of DNA fiber mapping is 1-2 Kb. The figure illustrates three probes ( a, b and c) simultaneously within 50 kb region.


    Fiber FISH

    Figure 5.

    Comparison of different resolutions between interphase FISH and fiber FISH. In the interphase nucleus (IN), the 3,000Kb region appears as a large spot, Chromatin fiber (CF) displays the same region as three separate domains.

References:
  • Liu L, Wang L, Jia HP, et al.
    Structure and mapping of the human beta-defensin HBD-2 gene and its expression at sites of inflammation.
    Gene 222:237-244 1998.

  • Schmid C, Heng HHQ, Rubin C, et al.
    Sperm nuclear matrix of the PRM1-PRM2-TNP2 domain is independent of ALU methylation.
    Hum Mole Reproduction 7: 903-911 2001.

    For more references, please check out the publications in the reliability section.

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