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- Determine the status of the integration of constructs into a host genome
- Integration efficiency can be determined
- The chromosomal location of the constructs and the number of integrating sites can be determined
- Determine the specificity of integration: random or non-random integration in the host genome
- Determine the level of integrity a construct maintains after transfection.
- The size and number of the inserted constructs in the same insertion site can be determined
- Determine the correlation between integration sites and the level of gene expression
- Determine the stability of integration after varying periods of cell division
- Trace sub-populations of cells after integration
Figure 3: Examples of FISH analysis of an AML5 cell with rAAV insertions.
- Zheng CY, Baum BJ, Iadarola MJ, et al.
Genomic integration and gene expression by a modified adenoviral vector.
NAT BIOTECHNOL 18: (2) 176-180 FEB 2000
- Omori F, Messner HA, Ye J, et al.
Non-targeted integration of recombinat adeno-associated virus in human cells detected by fluorescence in situ hybridization.
Human Gene Therapy 10: 537-543 1999.
For more references, please check out the publications in the reliability section.
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