FOR SHIPMENTS WITH TRANSGENIC MOUSE/RAT SPLEENS, CELL LINES AND PARAFFIN-EMBEDDED TISSUE SECTION SLIDES:
Please contact our Customer Service for shipment instructions by email at
FOR DNA SAMPLE PREPARATION:
20ug of purified DNA is required for each probe used in FISH mapping. Please adjust the concentration of the DNA sample to approximately 1ug/ul.
DNA should be purified by QIAGEN kits or other comparable methods. For DNA preparation (plasmid, phage and YAC DNA), detailed protocols can be found from the following reference:
Heng, HHQ and Tsui, L-C. (1994). chapter 4: FISH detection on DAPI-banded chromosomes. In Methods in Molecular Biology: In situ hybridization protocols, Ed Choo KHA, p.35-49. Humana Press, Totowa, NJ.
Please attach a gel picture, indicating the size of insert and vector for your DNA samples, and corresponding markers with volumes loaded.
DNA samples can be shipped at room temperature:
Ice is not required
Please mark on the commercial invoice and air waybill: "For research use only, non-hazardous. Value $1.00"
FOR ANTIBODY SAMPLE PREPARATION:
For detection on human or mouse cells (eg. Hela cell or mouse L-cell), slides will be provided by us.
If a special type of cell line is required for your antibody detection (eg. one specific antibody for positive and negative controls, specific tumor cells, transgenic or knock-out cell lines), the cell line can be cultured by us and slide preparation can be performed by us subject to a service charge.
Please send NON-diluted antibodies by request as follows:
10-20ul for primary polyclonal Ab or 200-500ul for primary monoclonal Ab.
A fact sheet for primary antibody for both commercial product and self-made Ab.
A suggested Ab dilution used in Western Blot hybridization.
